Anti-BARD1 Antibody Picoband®

SKU: A01646-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BARD1

Specificity:

Background: BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human BARD1 recombinant protein (Position: P709-A758).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Daza-Martin, M., Starowicz, K., Jamshad, M., Tye, S., Ronson, G. E., MacKay, H. L., Chauhan, A. S., Walker, A.K., Stone, H. R., Beesley, J. F. J., Coles, J. L., Garvin, A. J., Stewart, G. S., McCorvie, T. J., Zhang, X., Densham, R. M., Morris, J. R. Isomerization of BRCA1-BARD1 promotes replication fork protection. Nature 571: 521-527, 2019. 2. Dong, Y., Hakimi, M.-A., Chen, X., Kumaraswamy, E., Cooch, N. S., Godwin, A. K., Shiekhattar, R. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair. Molec. Cell 12: 1087-1099, 2003. 3. Irminger-Finger, I., Leung, W.-C., Li, J., Dubois-Dauphin, M., Harb, J., Feki, A., Jefford, C. E., Soriano, J. V., Jaconi, M., Montesano, R., Krause, K.-H. Identification of BARD1 as mediator between proapoptotic stress and p53-dependent apoptosis. Molec. Cell 8: 1255-1266, 2001.

Uniprot ID: Q99728

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 87 kDa

Calculated Molecular Weight: 141435 MW

Gene ID: 580

Protein Name: BRCA1-associated RING domain protein 1

Gene Full Name: BRCA1 associated RING domain 1

Synonyms: BARD 1; BARD1; BRCA1 associated RING domain 1