Anti-ATG4C Antibody Picoband®

SKU: A09728-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATG4C

Specificity:

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

Immunogen: E.coli-derived human ATG4C recombinant protein (Position: M1-L457).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Agrotis, A., Pengo, N., Burden, J. J., Ketteler, R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15: 976-997, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 12/16/2020. 3. Li, M., Hou, Y., Wang, J., Chen, X., Shao, Z.-M., Yin, X.-M. Kinetics comparisons of mammalian Atg4 homologues indicates selective preferences toward diverse Atg8 substrates. J. Biol. Chem. 286: 7327-7338, 2011.

Uniprot ID: Q96DT6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 67169 MW

Gene ID: 84938

Protein Name: Cysteine protease ATG4C

Gene Full Name: autophagy related 4C cysteine peptidase

Synonyms: Autophagin-3; ATG4C; APG4C; APG4 C; AUTL1; AUTL3; APG4-C; HsAPG4C