Anti-Arginase-2, mitochondrial Arg2 Antibody Picoband®

SKU: PA1805

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Gene Name: ARG2

Specificity: No cross reactivity with other proteins.

Background: ARG2 (arginase, type II) encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al. (1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis, the ARG2 gene is mapped to 14q24.1-q24.3. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al. (1996) showed that ARG2 mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of ARG2 and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that ARG2 is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Arg2, different from the related mouse sequence by three amino acids and from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Deignan, J. L., Livesay, J. C., Yoo, P. K., Goodman, S. I., O'Brien, W. E., Iyer, R. K., Cederbaum, S. D., Grody, W. W. Ornithine deficiency in the arginase double knockout mouse. Molec. Genet. Metab. 89: 87-96, 2006. 2. Gotoh, T., Sonoki, T., Nagasaki, A., Terada, K., Takiguchi, M., Mori, M. Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line. FEBS Lett. 395: 119-122, 1996. 3. Vockley, J. G., Jenkinson, C. P., Shukla, H., Kern, R. M., Grody, W. W., Cederbaum, S. D. Cloning and characterization of the human type II arginase gene. Genomics 38: 118-123, 1996.

Uniprot ID: P78540

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 38578 MW

Gene ID: 384

Protein Name: Arginase-2, mitochondrial

Gene Full Name: arginase 2

Synonyms: ARG2; Arginase 2; mitochondrial; arginase; type II; Kidney type arginase; Non hepatic arginase; Type II arginase