Anti-Aquaporin 10/AQP10 Antibody Picoband®

SKU: PA2095

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: AQP10

Specificity: No cross reactivity with other proteins.

Background: AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3 (53%), 9 (52%), and 7 (43%). These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP (AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hatakeyama, S., Yoshida, Y., Tani, T., Koyama, Y., Nihei, K., Ohshiro, K., Kamiie, J.-I., Yaoita, E., Suda, T., Hatakeyama, K., Yamamoto, T. Cloning of a new aquaporin (AQP10) abundantly expressed in duodenum and jejunum. Biochem. Biophys. Res. Commun. 287: 814-819, 2001.

Uniprot ID: Q96PS8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 31 kDa

Calculated Molecular Weight: 31763 MW

Gene ID: 89872

Protein Name: Aquaporin-10

Gene Full Name: aquaporin 10

Synonyms: AQP-10; Aquaglyceroporin-10; Small intestine aquaporin; AQP10; AQPA_HUMAN