Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

SKU: PA1793

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat

Gene Name: NOL3

Specificity: No cross reactivity with other proteins.

Background: NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: 213-221, 2004. 2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998. 3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. Chem. 274: 10951-10962, 1999.

Uniprot ID: O60936

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 27 kDa

Calculated Molecular Weight: 23 kDa

Gene ID: 8996

Protein Name: Nucleolar protein 3

Gene Full Name: nucleolar protein 3

Synonyms: Apoptosis repressor with CARD; ARC; CARD2; MYP; NOL3; NOP; NOP30; Nucleolar protein 3; Nucleolar protein of 30 kDa