Anti-Apg7/ATG7 Antibody Picoband®

SKU: PA2261

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: ATG7

Specificity: No cross reactivity with other proteins.

Background: Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) 2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Note: Erratum: Science 341: 457 only, 2013. 3. Tanida, I., Tanida-Miyake, T., Ueno, T., Kominami, E. The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem. 276: 1701-1706, 2001.

Uniprot ID: O95352

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 78 kDa

Calculated Molecular Weight: 77960 MW

Gene ID: 10533

Protein Name: Ubiquitin-like modifier-activating enzyme ATG7

Gene Full Name: autophagy related 7

Synonyms: APG7 LIKE; APG7L; ATG7; Autophagy related protein 7; GSA7; hAGP7