Anti-AMPK alpha 1/PRKAA1 Antibody Picoband®

SKU: A00994-6

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PRKAA1

Specificity:

Background: 5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Immunogen: E.coli-derived human AMPK alpha 1/PRKAA1 recombinant protein (Position: D359-P539).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit. 2. Hawley SA, Davison M, Woods A, et al. (1996). Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase.. J. Biol. Chem. 271 (44): 27879–87.

Uniprot ID: Q13131

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 64-66 kDa

Calculated Molecular Weight:

Gene ID: 5562

Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1

Gene Full Name: protein kinase AMP-activated catalytic subunit alpha 1

Synonyms: AMPK; AMPK alpha; AMPK alpha 1; AMPK subunit alpha 1; AMPK1; AMPKa1; PRKAA1