Anti-ALG3 Antibody Picoband®

SKU: A04688

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ALG3

Specificity:

Background: Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase is an enzyme that, in humans, is encoded by the ALG3 gene. This gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosylation type Id (CDG-Id) characterized by abnormal N-glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human ALG3 recombinant protein (Position: I61-H438).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Denecke, J., Kranz, C., Kemming, D., Koch, H.-G., Marquardt, T. An activated 5-prime cryptic splice site in the human ALG3 gene generates a premature termination codon insensitive to nonsense-mediated mRNA decay in a new case of congenital disorder of glycosylation type Id (CDG-Id). Hum. Mutat. 23: 477-486, 2004. 2. Denecke, J., Kranz, C., von Kleist-Retzow, J. C., Bosse, K., Herkenrath, P., Debus, O., Harms, E., Marquardt, T. Congenital disorder of glycosylation type Id: clinical phenotype, molecular analysis, prenatal diagnosis, and glycosylation of fetal proteins. Pediat. Res. 58: 248-253, 2005. 3. Korner, C., Knauer, R., Stephani, U., Marquardt, T., Lehle, L., von Figura, K. Carbohydrate deficient glycoprotein syndrome type IV: deficiency of dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase. EMBO J. 18: 6816-6822, 1999.

Uniprot ID: Q92685

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50-55 kDa

Calculated Molecular Weight:

Gene ID: 10195

Protein Name: Dol-P-Man:Man

Gene Full Name: ALG3 alpha-1,3- mannosyltransferase

Synonyms: ALG3; CDGS4; D16Ertd36e; NOT; Not56; Not56 like protein; NOT56L