Anti-ALAS2/ASB Antibody Picoband®

SKU: A02525-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ALAS2

Specificity:

Background: Delta-aminolevulinate synthase 2 also known as ALAS2 is a protein that in humans is encoded by the ALAS2 gene. The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen: E.coli-derived human ALAS2/ASB recombinant protein (Position: M1-D190).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aoki, Y., Urata, G., Takaku, F. Delta-aminolevulinic acid synthetase activity in erythroblasts of patients with primary sideroblastic anemia. Acta Haemat. Jpn. 36: 74-77, 1973. 2. Astner, I., Schulze, J. O., van den Heuvel, J., Jahn, D., Schubert, W.-D., Heinz, D. W. Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans. EMBO J. 24: 3166-3177, 2005. 3. Astrin, K. H., Bishop, D. F. Assignment of human erythroid delta-aminolevulinate synthase (ALAS2) to the X chromosome. (Abstract) Cytogenet. Cell Genet. 51: 953-954, 1989.

Uniprot ID: P22557

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 87164 MW

Gene ID: 212

Protein Name: 5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene Full Name: 5'-aminolevulinate synthase 2

Synonyms: ALAS E; ALAS2; ALASE; ANH1; ASB; Delta ALA synthase 2; FLJ93603; XLSA