Anti-ABCE1 Antibody Picoband®

SKU: A04173

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ABCE1

Specificity:

Background: ATP-binding cassette sub-family E member 1 (ABCE1) also known as RNase L inhibitor (RLI) is an enzyme that in humans is encoded by the ABCE1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.

Immunogen: E.coli-derived human ABCE1 recombinant protein (Position: Q141-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aubry, F., Mattei, M.-G., Barque, J.-P., Galibert, F. Chromosomal localization and expression pattern of the RNase L inhibitor gene. FEBS Lett. 381: 135-139, 1996. 2. Bisbal, C., Martinand, C., Silhol, M., Lebleu, B., Salehzada, T. Cloning and characterization of a RNase L inhibitor: a new component of the interferon-regulated 2-5A pathway. J. Biol. Chem. 270: 13308-13317, 1995. 3. Diriong, S., Salehzada, T., Bisbal, C., Martinand, C., Taviaux, S. Localization of the ribonuclease L inhibitor gene (RNS4I), a new member of the interferon-regulated 2-5A pathway, to 4q31 by fluorescence in situ hybridization. Genomics 32: 488-490, 1996.

Uniprot ID: P61221

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 67 kDa

Calculated Molecular Weight: 67 kDa

Gene ID: 6059

Protein Name: ATP-binding cassette sub-family E member 1

Gene Full Name: ATP binding cassette subfamily E member 1

Synonyms: ABC38; ABCE1; HuHP68; OABP; Ribonuclease 4 inhibitor; RLI; RNase L inhibitor; RNASEL1; RNASELI; RNS4I