ALKBH1 antibody , Cat. # FNab00311

SKU: FNab00311

Size:100ug

Purification:Immunogen affinity purified

Form:liquid

Purity:?95% as determined by SDS-PAGE

Host:Rabbit

Clonality:polyclonal

Clone ID:

Isotype:IgG

Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)

Background:Dioxygenase that acts as a DNA demethylase(PubMed:18603530). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:18603530). Specifically demethylates DNA methylated on the 6th position of adenine(N(6)-methyladenosine) DNA. N(6)-methyladenosine(m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing(By similarity). Also able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation, but with low activity(PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites(PubMed:19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product(PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation(By similarity).

Immunogen:alkB, alkylation repair homolog 1(E. coli)

Synonyms:ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH

Observed MW:44 kDa

Uniprot ID:Q13686

Reactivity:Human, Mouse, Rat

Tested Application:ELISA, WB, IHC

Recommended dilution:WB: 1:200-1:2000; IHC: 1:20-1:200

Gene ID:238

Research Area:Metabolism