GeneBio Systems

AFP ELISA kit (Rat)

Name: AFP ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA153Ra
Price: 179900 JPY
Availability: InStock

SKU:SEA153Ra

¥179,900 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 17

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: AFP

Target Full Name: Alpha-Fetoprotein

Alternative Names: aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein

Target Species: Rat

Uniprot: P02773

Gene ID: -

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat AFP / Alpha-Fetoprotein

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.312-20ng/mL

Sensitivity: 0.114ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (AFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (AFP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (AFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (AFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (AFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fetoprotein (AFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Hepatology;

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Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats

Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver

Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes.

Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats

Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme

Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats

Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model

Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway

Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles

Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats