AF/LE Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114]

SKU: GBS-F09900

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:MHC II (I-A/I-E)

Target Synonym:H2-Ab1/Eb1;I-E beta MHC class II;MHC class II;MHC class II H2-IA-beta-psi;Major histocompatibility protein class II beta chain

Research Areas:Immunology;Innate Immunity

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Mouse

Application:FCM

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Rat IgG2b, κ

Clonality:Monoclonal

Clone NO.:M5/114

UNIProt ID:P14483;O78196;

Background:These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Mouse MHC class II protein

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID: