AF/LE Purified Anti-Mouse CD62L Antibody[MEL-14]

SKU: GBS-F10110

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD62L

Target Synonym:CD62L;CD62 antigen-like family member L;L-selectin;LAM-1;LECAM1;Lnhr;Ly-22;Ly22;Lymph node homing receptor;Sell

Research Areas:Cell Adhesion;Cell Biology;Costimulatory Molecules;Immunology;Innate Immunity

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Mouse

Application:FCM;Neut

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Rat IgG2a, κ

Clonality:Monoclonal

Clone NO.:MEL-14

UNIProt ID:P18337

Background:CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte 'rolling' on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:20343