AF/LE Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]

SKU: GBS-F11120

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD45R/B220

Target Synonym:B220

Research Areas:Cell Biology;Immunology;Inhibitory Molecules;Neuroscience;Neuroscience Cell Markers

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Mouse

Application:FCM;Cell Sep-Neg

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Rat IgM, κ

Clonality:Monoclonal

Clone NO.:RA3.3A 1/6.1

UNIProt ID:

Background:CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:

Dilution:

GeneID: