GeneBio Systems

ACTH ELISA kit (Rat)

Name: ACTH ELISA kit (Rat)
Brand: GeneBio Systems
SKU: CEA836Ra
Price: 159900 JPY
Availability: InStock

SKU:CEA836Ra

¥159,900 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 13

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ACTH

Target Full Name: Adrenocorticotropic Hormone

Alternative Names: Corticotropin; Adrenocorticotropin

Target Species: Rat

Uniprot: P01194

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Rat ACTH / Adrenocorticotropic Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 1.28-800pg/mL

Sensitivity: 0.52pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.

Research Area: Endocrinology;Hormone metabolism;

References Citing This Product: Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats.

Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats

Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level

Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages

Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats

Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression

Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression.

Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway

Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation

Mixture effects of azole fungicides on the adrenal gland in a broad dose range

Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium …