ACH ELISA kit (General species)

SKU: CEA912Ge

Size: 96Tests

# of Times Cited in literature: 19

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ACH

Target Full Name: Acetylcholine

Alternative Names: -

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species ACH / Acetylcholine

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 12.35-1,000pg/mL

Sensitivity: 5.06pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcholine (ACH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcholine (ACH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Acetylcholine (ACH). No significant cross-reactivity or interference between Acetylcholine (ACH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcholine (ACH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcholine (ACH) and unlabeled Acetylcholine (ACH) (Standards or samples) with the pre-coated antibody specific to Acetylcholine (ACH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcholine (ACH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Acetylcholine (ACH) in the sample.

Research Area: Signal transduction;Infection immunity;Neuro science;

References Citing This Product: Combined administration of D-galactose and aluminium induces Alzheimer-like lesions in brain

Neuroprotective effects of simvastatin and Cilostazol in L-methionine-induced vascular dementia in rats

Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction

Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protection

Caffeic acid phenethyl ester counteracts doxorubicin-induced chemobrain in Sprague-Dawley rats: Emphasis on the modulation of oxidative stress and …

Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and …

Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion

Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain …

Study on the mechanisms of Suaeda rigida polysaccharides on the heart inhibition and skeletal muscle promotion in the frog