5-Me-THF(5-Methyltetrahydrofolate) ELISA Kit

SKU: ELK8059

Size:96T

Reactivity:General

Alternative Names:5-Me-THFA; 5-Me-H4FA; 5-Me-THF; 5-Me-H4F; Levomefolic Acid; Metafolin; 5-Methyltetrahydrofolic Acid; L-Methylfolate

Assay Type:Competitive Inhibition

Sensitivity:0.44 ng/mL

Standard:90 ng/mL

Detection range:1.41-90 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Hematology;Reproductive science;Nutrition metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.

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