4-HNE(4-Hydroxynonenal) ELISA Kit

SKU: ELK9581

Size:96T

Reactivity:General

Alternative Names:4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

Assay Type:Competitive Inhibition

Sensitivity:0.38 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

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