Amplicon sequencing generally refers to the targeted sequencing of specific DNA regions that have been amplified by polymerase chain reaction (PCR) used for the study of specific genomic regions, such as gene panels or exons. Hot spots refer to specific genomic regions known for high mutation rates or frequent genetic alterations with frequent applications in cancer genomics, drug resistance studies, and genetic diseases. Together, a hot spots amplicon sequencing panel uses small genomic regions as targets in order to select non-overlapping amplicons.
