Vinculin Monoclonal Antibody

SKU: AN005300L

Size::25μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Shipping:The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Tags/Controls Antibodies

Abbreviation:Vinculin

Target Synonym:Vinculin;Metavinculin (MV);VCL

Research Areas:Cardiovascular;Signal Transduction;Isotype/Loading Controls;Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human;Mouse;Rat

Application:WB;IHC;IF

Isotype:IgG1

Clonality:Monoclonal

Clone NO.:4D4

UNIProt ID:P18206

Accession:

Background:Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific.

Concentration:1 mg/mL

Immunogen:Recombinant human Vinculin protein expressed by E.coli

Buffer:PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Purification method:Protein A/G Purification

Dilution:WB 1:2000-1:4000;IHC 1:200-1:400;IF 1:200-1:400

Calculated MW:124 kDa

ObservedMW:126 kDa