VB2(Vitamin B2) ELISA Kit

SKU: ELK8266

Size:96T

Reactivity:General

Alternative Names:Riboflavin; Riboflavine

Assay Type:Competitive Inhibition

Sensitivity:2.45 ng/mL

Standard:600 ng/mL

Detection range:9.38-600 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Nutrition metabolism;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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