TransNGS® Tn5 DNA Library Prep Kit for Illumina® (for 50ng DNA)

SKU: KP101-11

Product Details

TransNGS^® Tn5 DNA Library Prep Kit for Illumina® is a reagent kit designed for Illumina high-throughput sequencing platform, suitable for preparing DNA library from 50 ng initial sample. The resulting library can be used for single-end or paired-end sequencing. This kit employs an in vitro transposition technique, which can simultaneously complete DNA fragmentation and adapter ligation within 5 minutes. In comparison to conventional library preparation method that requires DNA fragmentation, end

repair, and adapter ligation, the in vitro transposition technique significantly shortens library construction time and simplifies the operation.

Features

• Time saving and simple operation 

• Require small amount of DNA 

Applications

Prepare short fragment DNA library for Illumina high-throughput sequencing platform from purified dsDNA

Storage

at -18℃ or below for one year

Shipping

Dry Ice (-70℃)

Product Composition

Component	KP101-11 (12 rxns)	KP101-03 (96 rxns)
Tn5-50 Enzyme Mix	24 μl	192 μl
5×Insertion Buffer	75 μl	600 μl
2×Tn5 Digestion Mix	375 μl	4×750 μl
TransNGS® Tn5 Library Amplification SuperMix (2×)	300 μl	4×600 μl
Library Elution Buffer	1.2 ml	2×5 ml
Nuclease-free Water	1 ml	5 ml

References

1 Pei H, Teng W, Gao L, et al. Low-affinity SPL binding sites contribute to subgenome expression divergence in allohexaploid wheat[J]. Science China Life Sciences, 2023.(IF 10.37)