Simian OPG(Osteoprotegerin) ELISA Kit

SKU: ELK11547

Size:96T

Reactivity:Simian

Alternative Names:TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

Assay Type:Sandwich

Sensitivity:0.058 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Cytokine;Metabolic pathway;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

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