Sheep T4(Thyroxine) ELISA Kit

SKU: ELK0549

Size:96T

Reactivity:Sheep

Alternative Names:3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Assay Type:Competitive Inhibition

Sensitivity:1.83 ng/mL

Standard:300 ng/mL

Detection range:4.69-300 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2.5h

Research Area:Endocrinology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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