Sheep Interferon Gamma, IFN-gamma ELISA Kit

SKU: E0049Sh

Size:96 Tests-1 Kit

Background:Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

Research_area:Immunology; Stem Cells; Cancer

Applications:ELISA

Target_protein:IFN-gamma

Reactivity:Sheep

Assay type:Sandwich

Sensitivity:2.12ng/L

Detection range:5-300ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Interferon gamma

Species:Sheep

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P17773

MASS(Da):19,370

GeneID:443396

Synonyms:IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Gene names:IFNG

Standard solution:320ng/L