GeneBio Systems

SDC1 ELISA kit (Rat)

Name: SDC1 ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEB966Ra
Price: 881.00 GBP
Availability: InStock

SKU:SEB966Ra

£881.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 20

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SDC1

Target Full Name: Syndecan 1

Alternative Names: CD138; SDC; SYND1; Syndecan Proteoglycan 1

Target Species: Rat

Uniprot: P26260

Gene ID: 25216

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat SDC1 / Syndecan 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 1.56-100ng/mL

Sensitivity: 0.54ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 1 (SDC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 1 (SDC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Syndecan 1 (SDC1). No significant cross-reactivity or interference between Syndecan 1 (SDC1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 1 (SDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 1 (SDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 1 (SDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 1 (SDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;Immunodeficiency;

References Citing This Product: Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction

Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats

Damage of the endothelial glycocalyx in chronic kidney disease

Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury

Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding

Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.

Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury

Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats

Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.

Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis

Experimental models of endotheliopathy: impact of shock severity