Recombinant Vaccinia virus Late transcription elongation factor H5 (VACWR103)

SKU: P07242

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P07242

Gene Names: VACWR103

Alternative Name(s): (Viral late gene transcription factor 4 )(VLTF-4)

Abbreviation: Recombinant Vaccinia virus VACWR103 protein

Organism: Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Source: Mammalian cell

Expression Region: 1-203aa

Protein Length: Full Length

Tag Info: C-terminal hFc1-tagged

Target Protein Sequence: MAWSITNKADTSSFTKMAEIRAHLKNSAENKDKNEDIFPEDVIIPSTKPKTKRATTPRKPAATKRSTKKEEVEEEVVIEEYHQTTEKNSPSPGVSDIVESVAAVELDDSDGDDEPMVQVEAGKVNHSARSDLSDLKVATDNIVKDLKKIITRISAVSTVLEDVQAAGISRQFTSMTKAITTLSDLVTEGKSKVVRKKVKTCKK

MW: 51.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Involved in the co-transcriptional or post-transcriptional endoribonucleolytic cleavage that generates sequence-homogeneous 3' ends during late transcription. Involved in postreplicative transcription elongation on intermediate and late genes (Probable). Also involved in DNA replication and in multiple steps of virion morphogenesis. Required both for inclusion of virosoplasm into crescents as well as for maturation of immature virions (IV) into mature virions (MV).

Reference: "Purification and properties of the vaccinia virus mRNA processing factor." D'Costa S.M., Bainbridge T.W., Condit R.C. J. Biol. Chem. 283: 5267-5275(2008)

Function: