Recombinant SMC1A Monoclonal Antibody

SKU: AN301986L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:SMC1A

Target Synonym:SMC;CDLS;KIAA;SMC1L;SMC1A;CDLS2;DXS423E;SB1.8;SMC1;SMC1L1;SMC1alpha;SMCB;KIAA0178;Chromosome segregation protein SmcB;MGC138332;Segregation of mitotic chromosomes 1;SMC protein 1A;SMC1 (structural maintenance of chromosomes 1 yeast) like 1;SMC1 structural maintenance of chromosomes 1 like 1;SMC-1A;SMC-1-alpha;Structural maintenance of chromosomes 1A;Structural maintenance of chromosomes protein 1A

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A706

UNIProt ID:Q14683

Accession:

Background:he cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase. RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase. RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis. RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis. In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab.

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000-1:2000

Calculated MW:141 kDa

ObservedMW:160 kDa