Recombinant Smac/Diablo Monoclonal Antibody

SKU: AN301658L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Smac/Diablo

Target Synonym:DFNA;DIABLO;DFNA64;SMAC;0610041G12Rik;DBLOH;DBOH;Diablo homolog;Diablo homolog (Drosophila);Diablo homolog mitochondrial;Diablo IAP binding mitochondrial protein;Diablo like protein;DIABLO S;Direct IAP binding protein with low pI;Direct IAP-binding protein with low pI;FLJ10537;FLJ25049;mitochondrial;Mitochondrial Smac protein;Second mitochondria derived activator of caspase;Second mitochondria-derived activator of caspase;SMAC 3;Smac protein;SMAC3

Research Areas:Cell Biology;Cancer;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF;FCM;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A361

UNIProt ID:Q9NR28

Accession:

Background:Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Concentration:1 mg/mL

Immunogen:Recombinant human Smac/Diablo fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;IHC 1:50-1:100;IF 1:500-1:1000;FCM 1:50-1:100;IP 1:25-1:100

Calculated MW:27 kDa

ObservedMW:21 kDa