Recombinant Rhesus Macaque TNF-Alpha protein

SKU: CD00021

Size:5 ug

Background:Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Rhesus macaque and human TNF-α show approximately 98 % homology at the amino acid level. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rhesus Macaque Tumor Necrosis Factor-alpha

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cytotoxicity assay using Mouse L929 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 17 IU/mg in the presence of actinomycin D.

Synonyms:TNF-α, TNF-a, TNF-alpha, TNFA, TNFSF2,Monkey

Endotoxin:Less than 1 EU/μg of rRhTNF-α as determined by LAL method.

Storage:-20ºC

Sequence:VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL

uniprot accession:P79337

Molecular weight:Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.