Recombinant Rhesus Macaque IL-4 protein

SKU: CD00036

Size:5 ug

Background:Interleukin-4 (IL-4) is a pleiotropic cytokine mainly produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Additionally, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rhesus macaque IL-4 shares about 40 % ~ 45 % a.a. sequence identity with human, mouse, rat IL-4 and they are species-specific in their activities.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rhesus Macaque Interleukin-4

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than 1. ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:IL-4, IL4,EGK_16838,Monkey

Endotoxin:Less than 1 EU/μg of rRhIL-4 as determined by LAL method.

Storage:-20ºC

Sequence:HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS

uniprot accession:P79339

Molecular weight:Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.