Recombinant Rhesus Macaque IL-3 protein

SKU: CD00037

Size:2 ug

Background:Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated Mouse mast cells, Mouse keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and Mouse IL-3 share low homology.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rhesus Macaque Interleukin-3

Biological activity:Assay #1: Fully biologically active when compared to standard. The ED5 as determined by the dose-dependent stimulation of the proliferation of Mouse NFS-6 cells is less than 5. ng/ml, corresponding to a specific activity of > 2 × 15 IU/mg.Assay #2: The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 17 IU/mg.

Synonyms:IL-3, EGK_16827,IL3,Monkey

Endotoxin:Less than 1 EU/μg of rRhIL-3 as determined by LAL method.

Storage:-20ºC

Sequence:APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ

uniprot accession:P2514

Molecular weight:Approximately 14. kDa, a single non-glycosylated polypeptide chain containing 124 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.