Recombinant Rhesus macaque B7-H4/VTCN1 Protein (His Tag)

SKU: PKSQ050083

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: B7-H4;VTCN1

Target Synonym: B7-H4;B7H4T-cell costimulatory molecule B7x;B7S1;B7S1VCTN1;B7XPRO1291;B7h.5;B7x;FLJ22418;Immune costimulatory protein B7-H4;Protein B7S1;T cell costimulatory molecule B7x;V-set domain containing T cell activation inhibitor 1;V-set domain-containing ;Vtcn1

Research Areas:

Conjugation:

Target Species: Rhesus macaque

Expression Host: HEK293 Cells

Application: BLI

Fusion tag: C-His

UNIProt ID: F7B770

Accession: F7B770

Background: B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Concentration:

Activity: Loaded Recombinant Rhesus Macaque B7-H4 on HIS1K Biosensor, can bind Human B7-H4 mAb with an affinity constant of 0.578 pM as determined in BLI assay.

Sequence: Phe29-Ala258

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26.4 kDa

ObservedMW: 40-60 kDa