Recombinant Rat SCF/c-Kit Ligand Protein (Human Cells, His Tag)

SKU: PKSR030459

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: SCF;c-Kit Ligand

Target Synonym: FPH2;KIT ligand;KITLG;KL-1;Kitl;MGF;MGFSHEP7;Mast cell growth factor;SCF;SFc-Kit ligand;SLF;Stem Cell Factor;c-kit Ligand;steel factor

Research Areas:

Conjugation:

Target Species: Rat

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P21581

Accession: P21581

Background: SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.

Concentration:

Activity: Not validated for activity

Sequence: Gln26-Ala189

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.4 kDa

ObservedMW: 23-40 kDa