Recombinant Rat NT-proBNP protein (GST tag)

SKU: PDER100173

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:NT-proBNP

Target Synonym:ANFB_RAT;Brain natriuretic factor prohormone;Gamma-brain natriuretic peptide;Iso-ANP;Natriuretic peptides B;Nppb;preproBNP;proBNP

Research Areas:Neuroscience

Conjugation:

Target Species:Rat

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P13205

Accession:P13205

Background:Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis

Concentration:

Activity:Not validated for activity

Sequence:His27-Arg76

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:30.4 kDa

ObservedMW:30 kDa