Recombinant Rat IL-7 protein

SKU: CD00100

Size:2 ug

Background:Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and Mouse IL-7.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-7

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse 2E8 cells is less than 2. ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:IL-7, IL7,Rat

Endotoxin:Less than 1 EU/μg of rRtIL-7 as determined by LAL method.

Storage:-20ºC

Sequence:DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI

uniprot accession:P56478

Molecular weight:Approximately 14. 9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.