Recombinant Rat IL-2 protein

SKU: CD00108

Size:5 ug

Background:IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-2, Liquid

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse CTLL-2 cells is less than .2 ng/ml, corresponding to a specific activity of > 5. × 16 IU/mg.

Synonyms:IL-2, IL2,Rat

Endotoxin:Less than 1 EU/ug of rRtIL-2, Liquid as determined by LAL method.

Storage:-20ºC

Sequence:APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ

uniprot accession:P1718

Molecular weight:Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.

Formulation:

Reconstitution:A .2 μm filtered concentrated sterile solution in 1 mM sodium citrate, pH 4., 3 % glycerol.