Recombinant Rat IL-1α/IL-1F1 Protein(Trx Tag)

SKU: PDER100132

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Cytokines

Abbreviation:IL-1α/IL-1F1

Target Synonym:interleukin;Hematopoietin;IL1F1hematopoietin;IL1F;IL-1F;alpha;BAF;Hematopoietin-1;IL1;IL1 alpha;IL-1 alpha;IL1-A;IL-1A;IL1-ALPHA;IL1F1;IL-1F1;IL1F1hematopoietin-1;interleukin 1;interleukin-1 alpha;LAF;LEM;preinterleukin 1 alpha;pro-interleukin-1-alpha;Il1a;FAF;Hematopoietin 1;IL 1 alpha;IL 1A;IL1;IL1 ALPHA;IL1F1;Interleukin 1 alpha;LAF;LEM;Preinterleukin 1 alpha;Il1a

Research Areas:

Conjugation:

Target Species:Rat

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P16598

Accession:P16598

Background:IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T-and B-cells) immune systems. Cytokines can be classified into two groups: pro-and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

Concentration:

Activity:Not validated for activity

Sequence:Ser115-Ser270

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:37 kDa

ObservedMW:35 kDa