Recombinant Rat IGFBP-3 protein (His tag)

SKU: PDMR100028

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:IGFBP-3

Target Synonym:IBP-3;IGF-binding protein 3;IGFBP-3;Igfbp3;Insulin-like growth factor-binding protein 3

Research Areas:Signal Transduction;Cardiovascular;

Conjugation:

Target Species:Rat

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:P15473

Accession:P15473

Background:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Gln292

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:32.0 kDa

ObservedMW:42 kDa