Recombinant Rat IGF-1 protein

SKU: CD00116

Size:10 ug

Background:The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature rat IGF-1 shares 96 % a.a. sequence identity with human IGF-1 and exhibits cross-species activity.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Insulin-like Growth Factor 1

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:IGF-1,IGF,IGF1, Rat

Endotoxin:Less than 1 EU/μg of rRtIGF-1 as determined by LAL method.

Storage:-20ºC

Sequence:GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA

uniprot accession:P825

Molecular weight:Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 7 amino acid residues.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.