Recombinant Rat IFN- Gamma protein

SKU: CD00117

Size:20 ug

Background:Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with mouse IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interferon-gamma

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by an anti-viral assay using Mouse L929 cells infected with encephalomyocarditis (EMC) virus is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:IFN-γ, IFNG2, If2f,IFNG,Rat

Endotoxin:Less than 0.1 EU/μg of rRtIFN-γ as determined by LAL method.

Storage:-20ºC

Sequence:QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC

uniprot accession:P1581

Molecular weight:Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and .5 % Tween-8.