Recombinant Rat GM-CSF protein

SKU: CD00121

Size:5 ug

Background:Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse FDC-P1 is less than .1 ng/ml, corresponding to a specific activity of > 1. × 18 IU/mg.

Synonyms:GM-CSF,Gm-csf, Gmcsf,CSF2, Rat

Endotoxin:Less than 1 EU/μg of rRtGM-CSF as determined by LAL method.

Storage:-20ºC

Sequence:APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK

uniprot accession:P4875

Molecular weight:Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.