Recombinant Rat CINC-1/CXCL1 protein

SKU: CD00136

Size:5 ug

Background:Rat CXCL1, also known as CINC-1, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 1-1 ng/ml.

Synonyms:CINC-1/CXCL1, CINC-1, Gro1,Rat

Endotoxin:Less than 1 EU/μg of rRtCINC-1/CXCL1 as determined by LAL method.

Storage:-20ºC

Sequence:APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK

uniprot accession:P1495

Molecular weight:Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 15 mM NaCl.