Recombinant Rat AMPK alpha2 Protein (His Tag)

SKU: PDER100146

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:AMPK alpha2

Target Synonym:5'-AMP-activated protein kinase catalytic subunit alpha-2;AAPK2;ACACA kinase;AMPK alpha 2 chain;AMPK subunit alpha-2;AMPK2;AMPKa2;AMPKalpha2;Acetyl-CoA carboxylase kinase;HMGCR kinase;Hydroxymethylglutaryl-CoA reductase kinase;PRKAA;PRKAA2;Protein kinase

Research Areas:

Conjugation:

Target Species:Rat

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q09137

Accession:Q09137-1

Background:The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Concentration:

Activity:Not validated for activity

Sequence:Tyr16-Val195

Purity: > 85% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:20.0 kDa

ObservedMW:22.3 kDa