Recombinant Rat AIF-M1 protein (His tag)

SKU: PDER100205

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:AIF-M1

Target Synonym:AIFM1;Apoptosis inducing factor;Apoptosis inducing factor 1;CMTX4;COWCK;COXPD6;Harlequin;PDCD 8;Programmed cell death 8;Striatal apoptosis inducing factor;mitochondrial

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling;Cancer;Metabolism;Neuroscience

Conjugation:

Target Species:Rat

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q9JM53

Accession:Q9JM53

Background:Probable oxidoreductase that has a dual role in controlling cellular life and death, during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Concentration:

Activity:Not validated for activity

Sequence:Arg 150-Val 299

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:16.4 kDa

ObservedMW:18 kDa