Recombinant Porcine IL-1RA protein

SKU: CD00154

Size:5 ug

Background:IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the co-receptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The porcine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Porcine IL-1RA shares 86 % and 87 % a.a. sequence homology with bovine and canine IL-1RA.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Porcine Interleukin-1 Receptor Antagonist Protein

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inhibiting IL-1α-dependent proliferation of Mouse D1S cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 14 IU/mg in the presence of 75 pg/ml rPoIL-1α.

Synonyms:IL-1RA,IRAP1, IL1RN,Porcine

Endotoxin:Less than 0.1 EU/μg of rPoIL-1RA as determined by LAL method.

Storage:-20ºC

Sequence:HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ

uniprot accession:Q6PUJ3

Molecular weight:Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.