Recombinant Phospho-EGFR (Tyr1173) Monoclonal Antibody

SKU: AN302101L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Phospho-EGFR (Tyr1173)

Target Synonym:PIG;HER;Proto-oncogene c-ErbB;Receptortyrosine-protein kinase erbB;Receptor tyrosine-protein kinase erbB;NISBD;EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA;ErbB-1;Receptortyrosine-protein kinase erbB-1;Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1; NISBD2;epidermal growth factor receptor;ERBB;ERBB1;HER1;mENA;NISBD2;PIG61;Avian erythroblastic leukemia viral (v erb b) oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog);Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian);ErbB1;ERBB;erb-b2 receptor tyrosine kinase 1;Errp;Oncogen ERBB;Receptor tyrosine protein kinase ErbB 1;SA7;Species antigen 7;Urogastrone;v-erb-b Avian erythroblastic leukemia viral oncogen homolog;wa2;Wa5

Research Areas:Signal Transduction;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A825

UNIProt ID:P00533

Accession:

Background:The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand bindingresults in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation.Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the activestate enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domainof PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. TheGRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide adocking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specificserine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylatedby CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000;IF 1:1000

Calculated MW:134 kDa

ObservedMW:170 kDa