Recombinant Mouse TNF-α protein (His tag)

SKU: PDEM100273

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Cytokines

Abbreviation:TNF-α

Target Synonym:Cachectin;DIF;Membrane Form;TNF-Alpha;TNF-a;TNF-alpha;TNFSF2;TNFalpha;Tnf;Tnfa;Tnfsf1a;Tnfsf2;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2

Research Areas: Cancer;Cardiovascular;Metabolism Immunology;Signal transduction

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P06804

Accession:P06804

Background:Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

Concentration:

Activity:Not validated for activity

Sequence:Leu 80–Leu 235

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:17.1 kDa

ObservedMW:17 kDa