Recombinant Mouse SerpinD1/HCF2 Protein (His Tag)

SKU: PKSM041140

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: SerpinD1;HCF2

Target Synonym: HC-II;Heparin cofactor 2;Heparin cofactor II;Protease inhibitor leuserpin-2;Serpin D1

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P49182

Accession: P49182

Background: SerpinD1, also known as heparin cofactor II(HC-II), is a member of Serpin superfamily of the serine proteinase inhibitors. It is a single chain glycoprotein with a size of 66.5 kDa and is secreted from hepatocytes. HC-II acts as a thrombin inhibitor in the coagulation cascade, in a glycosaminoglycan-dependent pathway using the release of a sequestered hirudin-like N-terminal tail for interaction with thrombin. This serpin belongs to multiple member group V2 of vertebrate serpin classification. It has been suggested that HC-II is a predictor of decreased atherosclerosis in the elderly and protective against atherosclerosis in mice. HCII can used as a predictive biomarker and therapeutic target for atherosclerosis.

Concentration:

Activity: Not validated for activity

Sequence: Glu24-Ser478

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 5% Mannitol, 0.06% Tween 80, pH8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 53.1 kDa

ObservedMW: 70-90 kDa